ProDx CT/NG/UU DNA qPCR Detection Kit
Manufactured by Shanghai Promega Biological Products, Ltd.

The ProDx CT/NG/UU DNA qPCR Detection Kit is a four-color multiplex fluorescent probe based on the TaqMan® real-time PCR assay system. The assay can specifically detect Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Ureaplasma urealyticum (UU) DNA and an endogenous internal control (IC) that is a highly conserved sequence region in human β-globin gene (HBB) in one reaction. The internal control is designed to control the effectiveness in sample collection, DNA extraction and PCR amplification. The amplification system also includes dUTP and Uracil N-Glycosylase (UNG) to minimize PCR carryover contamination. The amplified CT, NG, UU and IC DNA fragments are detected in CY5, FAM, ROX and JOE/VIC channels, respectively, with the Applied Biosystems® 7500 Real-Time PCR System.

• Efficient Design: Multiplex reaction detecting CT, NG, UU pathogens in one reaction.
• High Level of DNA Recovery: One-step DNA extraction method with Chelex®-100 removes inhibitors and maintains DNA.
• Sensitive Detection: Use the robust Promega GoTaq® Hot Start DNA Polymerase for room temperature setup.
• Simple Operation: One-tube PCR master mix design greatly reduces setup time.
• Reliable Results: Designed with human β-globin gene (HBB) endogenous internal control (IC) to control the effectiveness in sample collection, DNA extraction and PCR amplification process. The system also includes dUTP and Uracil N-Glycosylase (UNG) to minimize PCR product contamination.

For in vitro diagnostic use. This product is only available in China.

This product is intended for professional use only. Diagnostic results obtained using the product must be interpreted in conjunction with other clinical or laboratory data. Other specimen types have not been validated and may result in false positive or false negative results.

References

1. Boel, C.H.E. et al. (2005) Evaluation of conventional and real-time PCR assays using two targets for confirmation of the COBAS AMPLICOR CT/NG test for Neisseria gonorrhoeae in clinical samples. J. Clin. Microbiol. 43, 2231–5.
2. Tabrizi, S.N. et al. (2004) Evaluation of real time polymerase chain reaction assays for confirmation of Neisseria gonorrhoeae in clinical samples tested positive in the Roche Cobas Amplicor assay. J. Sex Transm. Infect. 80, 68–71.
3. Whiley, D.M. and Sloots, T.P. (2005) Comparison of three in-house multiplex PCR assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis using real-time and conventional detection methodologies. J. Pathology 37, 364–70.
4. Tang, J. et al. (2011) Novel multiplex real-time PCR system using the SNP technology for the simultaneous diagnosis of Chlamydia trachomatis, Ureaplasma parvum and Ureaplasma urealyticum and genetic typing of serovars of C. trachomatis and U. parvum in NGU. J. Molecular and Cellular Probes 25(1), 55–9.