Eastep® RT Master Mix
Manufactured by Shanghai Promega Biological Products, Ltd.
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Size
Catalog Number: LS2050
Catalog Number: LS2052
Catalog Number: LS2054
Eastep® RT Master Mix 是两步法Real Time RT-PCR用来合成第一链cDNA的最佳逆转录反应试剂。本产品为5x预混液,主要成份包含高效率逆转录酶M-MLV、MgCl2、dNTP、Oligo(dT)15 Primer、Random Primers、RNase inhibitor和反应缓冲液,只需添加模板RNA和水即可迅速地开始反应。
本产品适用于合成Real Time PCR用短链目的片段cDNA模板,如需Real Time PCR检测,推荐与本公司生产的Eastep® qPCR Master Mix共同使用,能够进行高性能的基因表达分析,也适用于高效合成全长的cDNA模板,对扩增2kb左右的片段效率更高;此外,由于本产品中加入gDNA Remover能有效去除RNA模板中的gDNA污染,使实验数据更准确。
●完全预混型试剂,只需添加模板RNA和水即可;
●可在15min内高效合成Real Time PCR用短链目的片段cDNA模板,同时该产品也适用于合成全长的cDNA模板;
●使用了Oligo(dT)15 Primer和Random Primers两种逆转录引物,可对RNA的整个区域进行均一的逆转录反应;
●具有与Real Time PCR试剂的高兼容性,与本公司生产的Eastep® qPCR Master Mix共同使用时,能达到高的检测灵敏度;
●逆转录温度范围广,根据RNA模板的不同选择37-50℃进行实验;
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Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
本产品采用–20°C 运输。到货后,将全部组分储存于–20°C。
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
本产品采用–20°C 运输。到货后,将全部组分储存于–20°C。
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
本产品采用–20°C 运输。到货后,将全部组分储存于–20°C。
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