Eastep® RT Master Mix
Manufactured by Shanghai Promega Biological Products, Ltd.

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Catalog Number: LS2050

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Catalog Number: LS2052

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Catalog Number: LS2054

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Overview
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Eastep® RT Master Mix 是两步法Real Time RT-PCR用来合成第一链cDNA的最佳逆转录反应试剂。本产品为5x预混液,主要成份包含高效率逆转录酶M-MLV、MgCl2、dNTP、Oligo(dT)15 Primer、Random Primers、RNase inhibitor和反应缓冲液,只需添加模板RNA和水即可迅速地开始反应。
本产品适用于合成Real Time PCR用短链目的片段cDNA模板,如需Real Time PCR检测,推荐与本公司生产的Eastep® qPCR Master Mix共同使用,能够进行高性能的基因表达分析,也适用于高效合成全长的cDNA模板,对扩增2kb左右的片段效率更高;此外,由于本产品中加入gDNA Remover能有效去除RNA模板中的gDNA污染,使实验数据更准确。


●完全预混型试剂,只需添加模板RNA和水即可;
●可在15min内高效合成Real Time PCR用短链目的片段cDNA模板,同时该产品也适用于合成全长的cDNA模板;
●使用了Oligo(dT)15 Primer和Random Primers两种逆转录引物,可对RNA的整个区域进行均一的逆转录反应;
●具有与Real Time PCR试剂的高兼容性,与本公司生产的Eastep® qPCR Master Mix共同使用时,能达到高的检测灵敏度;
●逆转录温度范围广,根据RNA模板的不同选择37-50℃进行实验;

Protocols

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Specifications

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

本产品采用–20°C 运输。到货后,将全部组分储存于–20°C。

Certificate of Analysis

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

本产品采用–20°C 运输。到货后,将全部组分储存于–20°C。

Certificate of Analysis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

本产品采用–20°C 运输。到货后,将全部组分储存于–20°C。

Resources

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