Anti-HiBiT Monoclonal Antibody, XFD Fluorophore Conjugates
Fluorescent Antibodies for Direct Detection of HiBiT-Tagged Proteins
- Bright signal from fluorescent dyes with the same structure as Alexa Fluor® dyes
- Streamline flow cytometry workflows
- Preserve samples with fewer wash steps
Catalog Number:
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Catalog Number: N7221
Catalog Number: N7231
Simplify Flow Cytometry for HiBiT-Tagged Proteins
Anti-HiBiT Monoclonal Antibody, XFD Fluorophore Conjugates (same structure as Alexa Fluor® dyes), make flow cytometry analysis of HiBiT-tagged proteins more efficient. They eliminate the need for secondary antibodies, reducing long incubation times and wash steps that cause sample loss. Available in two different colors to enable multiplexing in both live-cell and fixed flow cytometry applications.
The 11aa HiBiT epitope tag provides researchers the flexibility to study protein dynamics in live cells, fixed cells and lysates with both sensitive bioluminescence detection and a high-affinity antibody. Anti-HiBiT Monoclonal Antibody, XFD Fluorophore Conjugates, further enhance the versatile HiBiT protein tagging system.
Detect HiBiT CRISPR Knock-In Cells via Flow Cytometry
HiBiT CRISPR knock-in (red) and parental control cells (blue) were stained with 1µg/ml of XFD647 (Panel A and Panel C) or XFD488 (Panel B and Panel D) Anti-HiBiT Monoclonal Antibody, XFD Fluorophore Conjugates. Samples were analyzed on a BD Accuri C6 Plus Flow Cytometer using the APC or FITC filters, respectively.
Alexa Fluor is a registered trademark of Thermo Fisher Scientific.
Simplify Your Workflow
Explore premade solutions to simplify your workflow—whether you need ready-to-use HiBiT-tagged protein vectors or prebuilt knock-in CRISPR cell lines designed for reliable expression and detection.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Anti-HiBiT Monoclonal Antibody XFD647 |
N722A | 1 × 100μl | 0.5μg/μl |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
This product and its derivatives may not be further transferred by the researcher and the purchased quantity of the product may be used only by the researcher, and then only for (1) research use, which may include drug discovery and development research; and (2) use in provision of services, that result in transfer of information or data only. No other commercial use of this product or derivatives is allowed. “Commercial use” means any and all uses of this product or derivatives by a party in exchange for money or other consideration, including, but not limited to, (1) use in further product manufacture; and (2) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researcher may not attempt to reverse engineer this product by any method including, without limitation, any method of sequencing any portion of the product.
With respect to any uses outside this label license, including, without limitation, any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Anti-HiBiT Monoclonal Antibody XFD488 |
N723A | 1 × 100μl | 0.5μg/μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
This product and its derivatives may not be further transferred by the researcher and the purchased quantity of the product may be used only by the researcher, and then only for (1) research use, which may include drug discovery and development research; and (2) use in provision of services, that result in transfer of information or data only. No other commercial use of this product or derivatives is allowed. “Commercial use” means any and all uses of this product or derivatives by a party in exchange for money or other consideration, including, but not limited to, (1) use in further product manufacture; and (2) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researcher may not attempt to reverse engineer this product by any method including, without limitation, any method of sequencing any portion of the product.
With respect to any uses outside this label license, including, without limitation, any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
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