Viral RNA/DNA Concentration and Extraction Kits for Wastewater
Direct Capture and Purification of Viral DNA/RNA From Wastewater
- Process 40ml input sample volume of wastewater
- Extract TNA in 2 hours or less and efficiently remove PCR inhibitors
- Choose from three formats to fit your sample throughput: Manual (A2991); Maxwell® automation (AS1831); or high-throughput, plate-based automation (AX9190)
- For new testers we offer a startup bundle including the vacuum manifold hardware (110V or 220V) for concentration plus a purification kit (see specs below)
- For additional voltage options, contact Technical Service
Complete Kit
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| A2991 | Wizard® Enviro TNA Kit | View Specifications | 25 preps | 价格查询 | |||
| AS1831 | Maxwell® RSC Enviro TNA Kit | View Specifications | 48 preps | 价格查询 | |||
| AX9190 | Maxwell® HT Environmental TNA Kit, Custom | View Specifications | 1 each | 价格查询 |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Start-Up Kit
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| A3060 | Wizard® Enviro Start-Up Bundle, 220V | View Specifications | 1 each | 价格查询 | |||
| A3080 | Maxwell® RSC Enviro Start-Up Kit, 220V | View Specifications | 1 each | 价格查询 |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Direct Capture of SARS-CoV-2 RNA in 2 Hours
The Enviro Total Nucleic Acid Kits for Wastewater include reagents and consumables to concentrate and purify total nucleic acids (TNA) in one convenient kit. Our unique vacuum-based direct capture method simplifies sample processing by concentrating and capturing viral TNA quicker than precipitation methods without the use of cumbersome ultracentrifugation, while achieving high and consistent yield. The entire process from sample to purified TNA takes less than 2 hours and is scalable and adaptable to your needs. The process also removes most PCR inhibitors, so the resulting TNA can be used directly for amplification of SARS-CoV-2 RNA using the SARS-CoV-2 RT-qPCR Kit for Wastewater.
We offer three kit formats based on sample throughput. Direct capture of nucleic acids is followed by purification using mini spin columns or benchtop automation using Maxwell® Instruments. A plate-based high-throughput kit is also available. An optional step can be included to extract nucleic acids from solid samples.
Choose A Format For Your Sample Throughput
Manual: Vacuum + Spin Column
The Wizard® Enviro Total Nucleic Acid Kit (A2991) allows you to get purified TNA in 90 minutes or less with a simple protocol. First, the TNA is directly captured from wastewater using the PureYield™ Midicolumn and Vac-Man® Laboratory Vacuum Manifold. Then, the lysate is further cleaned using PureYield™ Minicolumns. The recommended protocol starts from 40ml of wastewater and results in 40µl of TNA.
Maxwell® Automation: Vacuum + Maxwell® Extraction
The Maxwell® RSC Enviro Total Nucleic Acid Kit (AS1831) allows you to get purified TNA in 2 hours with vacuum-based direct capture of TNA followed by automated TNA extraction using Maxwell® Instruments. The Maxwell® RSC Instrument can process 1 to 16 samples, and the Maxwell® RSC 48 Instrument can process 1 to 48 samples in a single run. Viral TNA is first captured from wastewater using the PureYield™ Midicolumn and Vac-Man® Laboratory Vacuum Manifold. Then, the lysate is loaded into the Maxwell® RSC Instrument for walkaway RNA extraction. The recommended protocol starts from 40ml of wastewater and results in 40µl of TNA.
High-Throughput Concentration and Extraction: Plate-Based
High-throughput extraction solution designed for efficient, automated purification of TNA from environmental samples such as wastewater. This solution is compatible with upstream concentration methods—including magnetic particle-based approaches like Ceres Nanotrap® technology—which must be sourced separately. Our Field Support Scientists can help you implement or optimize automation of viral RNA extraction methods that meets your specific needs. Please contact us for more information.
Simple Direct Capture Protocol
The filter-based Direct Capture method allows rapid purification and concentration of viral nucleic acids from large volumes of wastewater samples.
Direct Capture Results in Higher Yield Than PEG/NaCl
Preliminary data shows the direct capture method resulted in 30 times higher SARS-CoV-2 RNA yield compared to PEG/NaCl. Wastewater samples were collected from a major US city.
Select Which Product is Right for You
Learn more about wastewater surveillance methods and tools.
| Cat.# | Name | Concentration Method | Input Sample Volume | Intended Throughput | Size | Format |
|---|---|---|---|---|---|---|
| A2991 | Wizard® Enviro TNA Kit | Vacuum-Based | 40ml | <16 | 25 preps | Manual Spin Column |
| AS1831 | Maxwell® RSC Enviro TNA Kit | Vacuum-Based | 40ml | 1–48 | 48 preps | Semi-Automated |
| AX9190 | Maxwell® HT Enviro TNA Kit | Varies | 10ml | >48 | 4 × 96 preps | High-Throughput |
| A3130 | Wizard® PureWater Kit | Any | Varies | <16 | 50 preps | Manual Spin Column |
| AS2110 | Maxwell® RSC PureWater Kit | Any | Varies | 1–48 | 48 preps | Semi-Automated |
What Customers Are Saying
“We are very happy to have implemented the large volume extraction kits in our analysis of wastewater for SARS-CoV-2. The integration of concentration and extraction steps has been instrumental in expediting our workflow. The kits also provide higher reproducibility between biological replicates compared to the method we were using previously.”
Jeseth Delgado Vela
Asst. Professor, Howard University
“We have tested the new Promega Wastewater Kit and were very pleased with results. In my side-by side comparison, the Promega Wastewater Kit has demonstrated way higher viral RNA recovery. We are planning to use it for all future wastewater work.”
Sergey Balashov
PCR Laboratory Director, EMSL Analytical
Have questions? We're here to help!
Contact Us
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Binding Buffer 1 (BBD) |
A2981 | 1 × 320ml | View Product |
Binding Buffer 2 (BBE) |
MC1501 | 1 × 30ml | View Product |
Protease Solution |
A144C | 1 × 13ml | View Product |
Column Wash 1 (CWE) |
A296A | 1 × 85.3ml | |
Column Wash 2 (RWA) |
A297A | 1 × 206ml | |
|
Nuclease-Free Water |
P1195 | 1 × 150ml | View Product |
|
PureYield™ Binding Columns |
A245A | 5 × 5 each | |
PureYield™ Minicolumns |
A247C | 1 × 25/pk | |
PureYield™ Collection Tubes |
A167C | 1 × 25/pk | |
|
Elution Tubes |
Z200C | 1 × 50/pk | |
Reservoir Extension Funnel |
A3041 | 1 × 25/pk | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Column Wash 1 (CWE) |
A296A | 2 × 85.3ml | |
Column Wash 2 (RWA) |
A297A | 2 × 206ml | |
|
Nuclease-Free Water |
P1195 | 1 × 150ml | View Product |
|
PureYield™ Binding Columns |
A245C | 12 × 4 each | |
Elution Tubes (0.5ml) |
AS620A | 1 × 50/pk | View Product |
Maxwell® RSC Cartridge (RSCJ) |
AS159A | 48 × 1 each | |
RSC Plungers |
AS1670 | 1 × 48/pack | View Product |
|
Elution Tubes |
Z200C | 1 × 50/pk | |
Binding Buffer 1 (BBD) |
A2981 | 2 × 320ml | View Product |
Binding Buffer 2 (BBE) |
MC1501 | 2 × 30ml | View Product |
Protease Solution |
A1442 | 1 × 30ml | View Product |
Reservoir Extension Funnel |
A3041 | 2 × 25/pk | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Protease Solution, Custom |
AX918A | 1 × 60ml | |
Cell Lysis Buffer (CLD) |
A173C | 1 × 160ml | View Product |
Resin |
A175B | 4 × 5.5ml | View Product |
Wash Buffer (WBA) |
A176C | 3 × 350ml | View Product |
CTAB Buffer |
MC1411 | 1 × 100ml | View Product |
25mM Tris-HCl (pH 8.0) |
A2641 | 2 × 60ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Wizard® Enviro TNA Kit |
A2991 | 1 × 25 preps | View Product |
|
Welch Vacuum Pump for Continental Europe Electrical |
A6722 | 1 × 1 each | |
|
Eluator™ Vacuum Elution Device |
A1071 | 1 × 4 each | View Product |
|
Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity |
A7231 | 1 × 1 each | View Product |
Vac-Man® Jr. Laboratory Vacuum Manifold, #10 Stopper |
A3110 | 1 × 1 each | View Product |
Polypropylene Vacuum Flask, 1,700ml |
AS1839 | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Maxwell® RSC Enviro TNA Kit |
AS1831 | 1 × 48 preps | View Product |
|
Welch Vacuum Pump for Continental Europe Electrical |
A6722 | 1 × 1 each | |
|
Eluator™ Vacuum Elution Device |
A1071 | 1 × 4 each | View Product |
|
Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity |
A7231 | 1 × 1 each | View Product |
Vac-Man® Jr. Laboratory Vacuum Manifold, #10 Stopper |
A3110 | 1 × 1 each | View Product |
Polypropylene Vacuum Flask, 1,700ml |
AS1839 | 1 × 1 each |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Resources
Software and Firmware
Other Resources
- Paper: A Direct Capture Method for Purification and Detection of Viral Nucleic Acid Enables Epidemiological Surveillance of SARS-Cov-2
- Wastewater-Based Epidemiology: Sewage Surveillance to Track COVID-19
- Blog Post: How A New Wastewater Surveillance Method Predicted COVID-19 Cases In Italy
- Method Comparison: Purification and Detection of SARS-CoV-2 Viral RNA from Wastewater
- Video Protocol: Setting up the Vacuum Pump with Vac-Man® Manifold
- Video Protocol: Direct Capture of Nucleic Acid from Wastewater using Wizard® Enviro Total Nucleic Acid Kit
- Video Protocol: Direct Capture of Nucleic Acid from Wastewater using Maxwell® RSC Enviro Total Nucleic Acid Kit
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Frequently Used With
Maxwell® RSC Instrument
Benchtop instrument for automated nucleic acid purification with integrated quantitation. For Research Use Only.
AS4500
Maxwell® RSC 48 Instrument
Benchtop instrument for automated nucleic acid purification of 1–48 samples. For Research Use Only.
AS8500
Vac-Man® Jr. Laboratory Vacuum Manifold
For rapid, effective nucleic acid purification. Process two samples at a time.
A7660, A3110
Eluator™ Vacuum Elution Device
Eliminates need for centrifugation when eluting DNA with PureYield™ Systems.
A1071
Vac-Man® Laboratory Vacuum Manifold
For fast, effective nucleic acid purification. Process up to 20 samples simultaneously.
A7231
Binding Buffer 1 (BBD)
Component of the Wizard® and Maxwell® RSC Enviro Total Nucleic Acid Kits available as a standalone item.
A2981
Binding Buffer 2 (BBE)
Component of the Wizard® and Maxwell® RSC Enviro Total Nucleic Acid Kits available as a standalone item.
MC1501
Protease Solution
Component of the Wizard® and Maxwell® RSC Enviro Total Nucleic Acid Kits available as a standalone item.
A1442
