Lumit® VEGF-A (Human) Immunoassay

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ELISA Alternative for VEGF-A Detection

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Lumit® VEGF-A (Human) Immunoassay

Quantify VEGF-A Release in Less Than 70 Minutes

Vascular Endothelial Growth Factor A (VEGF-A), a multifunctional cytokine, is important for normal development and the maintenance of the vascular and lymphatic systems. It is produced by many cells, including endothelial cells, macrophages and activated T cells. While VEGF-A plays a beneficial role in wound healing, its dysregulation contributes to tumorigenesis and vascular disease in various parts of the body.

The Lumit® VEGF-A Immunoassay quantitatively measures released VEGF-A in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer—the entire protocol is completed in less than 70 minutes. Use the assay directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit® VEGF-A Immunoassay Works

Primary antibodies to VEGF-A were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of VEGF-A, the subunits come together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to VEGF-A levels. The assay recognizes human VEGF-A, but not mouse or rat VEGF-A. It is specific for VEGF-A (121, 165 and 183) and does not cross-react with VEGF-B, -C and -D family members.

Schematic showing Lumit® Immunoassay concept.

Avoid Tedious Wash Steps with Simple One-Plate Protocol

Lumit® Immunoassay Workflow

Broad Linear Range of Lumit® VEGF-A Detection

The picomolar to nanomolar linear detection range of the Lumit® VEGF-A Immunoassay covers expected concentrations for most cell models.

Graph showing standard curve for Lumit® VEGF-A (Human) Immunoassay.

Specification

Lumit® VEGF-A Immunoassay

Dynamic Range

18.2–25,000pg/ml

Limit of Detection

3pg/ml (3 SD above background)

Minimal Detectable Dose (MDD)

2pg/ml (2 SD above background)

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

Featured Webinar

Optimizing the Lumit® IL-6 Immunoassay for High-Throughput Drug Screening

In this webinar, we share how to scale and optimize Lumit® Immunoassays for HTS applications. Learn how researchers used the Lumit® IL-6 Immunoassay in a screening campaign for antiviral drugs.

View On-Demand Webinar

 

Need to measure a different target?

View our growing portfolio of
Lumit® Immunoassays.

For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Resources

Articles

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