ProDx HSV-1/2 DNA qPCR Detection Kit
Manufactured by Shanghai Promega Biological Products, Ltd.

The ProDx HSV-1/2 DNA qPCR Detection Kit is a three color multiplex fluorescent probe based the TaqMan® real-time PCR assay system. The assay can specifically detect Herpes simplex virus (HSV)-1 and HSV-2 viral DNA and an endogenous internal control (IC) DNA that is a highly conserved region in the human β-globin gene (HBB) in one reaction. The internal control is designed to control the effectiveness of sample collection, DNA extraction and PCR amplification process. The system also includes dUTP and Uracil N-Glycosylase (UNG) to minimize PCR carryover contamination. The amplified HSV1, HSV2 and IC DNA fragments are detected in JOE, FAM and ROX channels, respectively, with the Applied Biosystems® 7500 Real-Time PCR System.

Performance Specifications
Sample Types: Urethral and cervical swabs.
Analytical Sensitivity: Detects >5 copies/µl of reference DNA.
Analytical Specificity: Negative against CT, NG, UU, HPV, HCMV and 12 other  microorganisms commonly found in the urethral and cervical area.
Precision: The intra- and inter-batch assay variation coefficients <5%.

• Efficient Design: Single-tube reaction detecting HSV-1 and HSV-2 and one internal control (IC).
• High Level of DNA Recovery: One-step DNA extraction method with Chelex®-100 removes inhibitors and maintains DNA.
• Sensitive Detection: Use the robust Promega GoTaq® Hot Start DNA Polymerase for room temperature setup.
• Simple Operation: One-tube PCR master mix design greatly reduces setup time.
• Reliable Results: Designed with human β-globin gene (HBB) endogenous internal control (IC) to control the effectiveness in sample collection, DNA extraction and PCR amplification process. The system also includes dUTP and Uracil N-Glycosylase (UNG) to minimize PCR product contamination.

For in vitro diagnostic use. This product is only available in China.

This product is intended for professional use only. Diagnostic results obtained using the product must be interpreted in conjunction with other clinical or laboratory data. Other specimen types have not been validated and may result in false positive or false negative results.

References

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4. Ramaswamy, M. et al. (2004) Diagnosis of genital herpes by real time PCR in routine clinical practice. Sex Transm Infect 80, 406–10.
5. Pandori, M.W. et al. (2006) Real-time PCR for detection of herpes simplex virus without nucleic acid extraction. BMC Infectious Diseases 6,1–9.
6. Corey, L. et al. (2005) Differentiation of herpes simplex virus types 1 and 2 in clinical samples by a real-time Taqman PCR assay. J Med Virol. 76, 350–5.
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