Anti-NanoLuc® Monoclonal Antibody
Detects NanoLuc® Luciferase and NanoLuc® Fusion Proteins
- Affinity-purified mouse monoclonal antibody
- Use in Western blotting and immunofluorescence
Catalog Number:
Size
Catalog Number: N7000
Antibody to Detect NanoLuc® Luciferase
Anti-NanoLuc® Monoclonal Antibody can be used to detect NanoLuc® Luciferase or NanoLuc® fusion proteins by Western blotting and immunofluorescence. The Anti-NanoLuc® Antibody is a protein A/G affinity-purified mouse monoclonal antibody.
NanoLuc® luciferase is a small (19.1kDa), stable reporter enzyme that generates a bright luminescence signal. The small size and bright signal make NanoLuc® a versatile reporter that is used in many protein analysis applications. NanoLuc® fusion proteins are used for protein stability analysis and in NanoBRET® target engagement and protein interaction applications.
Western Blot Results
The figure on the right shows an example Western blot using HEK293 cells transfected with CMV-based expression constructs encoding NanoLuc® fusion proteins. The actual mobility approximated the expected mobility for each fusion protein. For Western blotting, we recommend a concentration of 1µg/ml as a starting point for protocol optimization. Background bands are evident in all lanes, including HEK293 cell lysate, which lacks expression of a NanoLuc® fusion protein, but the NanoLuc® fusion bands are more prominent.
Detection of NanoLuc® fusion proteins expressed in HEK293 cells.
Immunofluorescence Results
Shown below are example immunofluorescent images of HeLa cells transfected with a CMV-based construct expressing CDK2-NanoLuc® fusion protein. The CDK2-NanoLuc® fusion protein is stained red, and nuclei are marked blue via Hoechst staining. We recommend a concentration of 1-7.5µg/ml for immunofluorescence optimization. No NanoLuc® staining is observed in HeLa cells transfected with only carrier DNA (mock).
CDK2-NanoLuc®
(3µg/ml Anti-NanoLuc®)
NanoLuc®
Hoescht
Merge
Mock (5µg/ml Anti-NanoLuc®)
NanoLuc®
Hoescht
Merge
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
Anti-NanoLuc® Monoclonal Antibody |
N700A | 1 × 100μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Related Products
Frequently Used With
NanoBRET® PPI Starter Systems
Live-cell protein:protein interaction assay. Includes vectors, controls and detection reagent for BRET-based PPI.
N1811, N1821
NanoBRET® Target Engagement Kinase Assays
Directly measure kinase-ligand binding affinity, occupancy and residence time in live cells.
N2520, N2521, N2540, N2501, N2500, N2530, N2600, N2601, N2620, N2621, N2630, N2631, N2640, N2641, N2650, N2651, NF1001, N2810, N2820, N2830, N2840, N2850, NF1200
Nano-Glo® Live Cell Assay
Measures NanoBiT® or NanoLuc® luminescence in living cells for up to 2 hours.
N2011, N2012, N2013
Nano-Glo® Luciferase Assay System
Add-mix-measure assay for NanoLuc® Luciferase with >2 hour half-life in most applications.
N1110, N1120, N1130, N1150
