PowerPlex® 16 System
Multiplex STR System for use in DNA Typing
- Amplify all 13 CODIS STR loci in one reaction
- Analyze minute quantities of DNA
- Size standard, amplification-grade water and master mix are included
Catalog Number:
Size
Catalog Number: DC6531
Catalog Number: DC6530
The PowerPlex® 16 System is a multiplex STR system for use in DNA typing, including paternity testing, forensic DNA analysis, human identity testing and tissue culture strain identification. This system allows co-amplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin): Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. One primer for each of the Penta E, D18S51, D21S11, TH01 and D3S1358 loci is labeled with fluorescein (FL); one primer for each of the FGA, TPOX, D8S1179, vWA and Amelogenin loci is labeled with carboxy-tetramethylrhodamine (TMR); and one primer for each of the Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818 loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE). All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane.
The PowerPlex® 16 System has undergone extensive validation efforts including internal validation, CODIS database validation and forensic casework validation. This system is optimized for use with 1ng of DNA; however, each lot performance tested to provide reproducible results with 0.5ng of DNA. Additional studies show interpretable results can be obtained with <0.5ng of DNA. The Internal Lane Standard 500 marker is used in each gel lane or capillary injection to increase the precision of analysis and allelic ladders for all 16 loci are provided to simplify interpretation. Panels and bins text files are required for use with GeneMapper® ID and GeneMapper® ID-X software and are available for download.
The PowerPlex® 16 System is compatible with the Applied Biosystems 3130 and 3130xl Genetic Analyzers. The PowerPlex® 4C Matrix Standard (Cat.# DG4800) is required for use of this system with the Applied Biosystems 3130 and 3130xl Genetic Analyzers.
Rapid Processing Solutions for PowerPlex®
The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.
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Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
2800M Control DNA |
DD710A | 1 × 25μl | 10ng/μl | View Product |
|
Internal Lane Standard 600 |
DG107A | 1 × 150μl | View Product | |
|
PowerPlex® 16 Allelic Ladder Mix |
DG600B | 1 × 50μl | ||
|
PowerPlex® 16 10X Primer Pair Mix |
DK711B | 1 × 250μl | ||
|
Gold ST★R 10X Buffer |
DM241C | 1 × 300μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
The purchase of this product does not convey a license to use AmpliTaq Gold® DNA polymerase. You should purchase AmpliTaq Gold® DNA polymerase licensed for the forensic and human identity field directly from your authorized enzyme supplier.
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
2800M Control DNA |
DD710A | 1 × 25μl | 10ng/μl | View Product |
|
Internal Lane Standard 600 |
DG107A | 4 × 150μl | View Product | |
|
PowerPlex® 16 Allelic Ladder Mix |
DG600B | 4 × 50μl | ||
|
PowerPlex® 16 10X Primer Pair Mix |
DK711B | 4 × 250μl | ||
|
Gold ST★R 10X Buffer |
DM241C | 4 × 300μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
The purchase of this product does not convey a license to use AmpliTaq Gold® DNA polymerase. You should purchase AmpliTaq Gold® DNA polymerase licensed for the forensic and human identity field directly from your authorized enzyme supplier.
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Software and Firmware
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