UDP-Glo™ Glycosyltransferase Assay
Universal Glycosyltransferase Activity Assay
- Easy, "add-incubate-read" protocol
- Detects any glycosyltransferase that uses UDP-sugar as a substrate
- Ideal for low-activity glycosyltransferases
Catalog Number:
Size
Add a UDP-sugar substrate
Catalog Number: V6961
Catalog Number: V6962
Catalog Number: V6963
Catalog Number: V6971
Catalog Number: V6972
Catalog Number: V6981
Catalog Number: V6982
Catalog Number: V6991
Catalog Number: V6992
Catalog Number: V7051
Catalog Number: V7052
Catalog Number: V7061
Catalog Number: V7062
A Simple, Bioluminescent Assay for Glycosyltransferase Activity
The UDP-Glo™ Glycosyltransferase Assay provides a homogeneous, single-reagent-addition method to rapidly detect UDP formation in glycosyltransferase reactions. After the glycosyltransferase reaction, an equal volume of UDP Detection Reagent is added to convert the UDP product to ATP and generate light in a luciferase reaction. The light generated is correlated to UDP concentration by using an UDP standard curve.
This assay is intended for use with purified glycosyltransferases that use UDP-sugar as a donor substrate and cannot be used with whole cells or cell extracts. However, glycosyltransferases purified from cell extracts using immunoprecipitation or affinity tag pull-down can be used in the UDP-Glo™ Assay.
Applications of the UDP-Glo™ Assay include profiling glycosyltransferase specificity for different sugars, screening ]library compounds for effects on glycosyltransferase activity, and detecting chemical compound glucuronidation during drug discovery.
Works with Most Glycosyltransferases
UDP-Glo™ Assay can be used to measure the activity of just about any glycosyltransferase (GT) that uses a UDP-sugar as a substrate. Here, we looked at GTs involved in glycan biosynthesis, post-translational modification and drug metabolism.
Detects Low Nanomolar Amounts of UDP; Ideal for Low-Activity Glycosyltransferases
The data to the left shows the linearity and sensitivity of the UDP-Glo™ Glycosyltransferase Assay. Here, a UDP standard curve was prepared over the indicated range of UDP concentrations in 25µl of 1X glycosyltransferase reaction buffer in a solid white 96-well plate. UDP-Glo™ Glycosyltransferase Assay was performed using 25µl of UDP Detection Reagent at room temperature. Luminescence was recorded using a GloMax® luminometer. Values represent the mean of four replicates. The table shows luminescence measured 1 hour after adding the UDP Detection Reagent, and signal-to-background ratios calculated for each concentration of the UDP standard curve.
Luminescent Signal is Stable Over Several Hours
The UDP-Glo™ Assay generates a highly stable luminescent signal with >80% signal remaining after 3 hours, allowing convenient batch-processing of assay plates.
The data here shows that the signal obtained with the UDP-Glo™ Assay is stable for several hours. To determine signal stability, luminescence was recorded every hour. The signal-to-background ratio did not change over the time measured (data not shown). RLU = relative light units.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
ATP Detection Substrate |
V363C | 1 × 1 vial |
Nucleotide Detection Buffer |
V696A | 1 × 5ml |
UDP-Glo™ Enzyme |
V697A | 1 × 20μl |
UDP, 10mM |
V698A | 1 × 100μl |
Enzyme Dilution Buffer |
V773A | 1 × 1.5ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
ATP Detection Substrate |
V363A | 1 × 1 vial |
Nucleotide Detection Buffer |
V696B | 1 × 10ml |
UDP, 10mM |
V698A | 1 × 100μl |
UDP-Glo™ Enzyme |
V697B | 1 × 40μl |
Enzyme Dilution Buffer |
V773A | 2 × 1.5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
ATP Detection Substrate |
V363B | 1 × 1 vial |
Nucleotide Detection Buffer |
V696C | 1 × 100ml |
UDP, 10mM |
V698A | 1 × 100μl |
UDP-Glo™ Enzyme |
V697C | 1 × 200μl |
Enzyme Dilution Buffer |
V773B | 1 × 15ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
UDP-GlcNAc, 100mM |
V707A | 1 × 50μl | View Product |
ATP Detection Substrate |
V363C | 1 × 1 vial | |
Nucleotide Detection Buffer |
V696A | 1 × 5ml | |
UDP-Glo™ Enzyme |
V697A | 1 × 20μl | |
UDP, 10mM |
V698A | 1 × 100μl | |
Enzyme Dilution Buffer |
V773A | 1 × 1.5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
ATP Detection Substrate |
V363A | 1 × 1 vial | |
UDP, 10mM |
V698A | 1 × 100μl | |
UDP-GlcNAc, 100mM |
V707A | 1 × 50μl | View Product |
Nucleotide Detection Buffer |
V696B | 1 × 10ml | |
UDP-Glo™ Enzyme |
V697B | 1 × 40μl | |
Enzyme Dilution Buffer |
V773A | 2 × 1.5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
ATP Detection Substrate |
V363C | 1 × 1 vial | |
Nucleotide Detection Buffer |
V696A | 1 × 5ml | |
UDP-Glo™ Enzyme |
V697A | 1 × 20μl | |
UDP, 10mM |
V698A | 1 × 100μl | |
UDP-GalNAc, 100mM |
V708A | 1 × 50μl | View Product |
Enzyme Dilution Buffer |
V773A | 1 × 1.5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
UDP, 10mM |
V698A | 1 × 100μl | |
|
ATP Detection Substrate |
V363A | 1 × 1 vial | |
Nucleotide Detection Buffer |
V696B | 1 × 10ml | |
UDP-GalNAc, 100mM |
V708A | 1 × 50μl | View Product |
UDP-Glo™ Enzyme |
V697B | 1 × 40μl | |
Enzyme Dilution Buffer |
V773A | 2 × 1.5ml |
SDS
Search for SDSCertificate of Analysis
Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
ATP Detection Substrate |
V363C | 1 × 1 vial | |
Nucleotide Detection Buffer |
V696A | 1 × 5ml | |
UDP-Glo™ Enzyme |
V697A | 1 × 20μl | |
UDP, 10mM |
V698A | 1 × 100μl | |
UDP-Glc, 100mM |
V709A | 1 × 50μl | View Product |
Enzyme Dilution Buffer |
V773A | 1 × 1.5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
ATP Detection Substrate |
V363A | 1 × 1 vial | |
Nucleotide Detection Buffer |
V696B | 1 × 10ml | |
UDP, 10mM |
V698A | 1 × 100μl | |
UDP-Glc, 100mM |
V709A | 1 × 50μl | View Product |
UDP-Glo™ Enzyme |
V697B | 1 × 40μl | |
Enzyme Dilution Buffer |
V773A | 2 × 1.5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
ATP Detection Substrate |
V363C | 1 × 1 vial | |
Nucleotide Detection Buffer |
V696A | 1 × 5ml | |
UDP-Glo™ Enzyme |
V697A | 1 × 20μl | |
UDP, 10mM |
V698A | 1 × 100μl | |
| UDP-Gal, 100mM | V717A | 1 × 50μl | View Product |
Enzyme Dilution Buffer |
V773A | 1 × 1.5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
ATP Detection Substrate |
V363A | 1 × 1 vial | |
Nucleotide Detection Buffer |
V696B | 1 × 10ml | |
UDP, 10mM |
V698A | 1 × 100μl | |
| UDP-Gal, 100mM | V717A | 1 × 50μl | View Product |
UDP-Glo™ Enzyme |
V697B | 1 × 40μl | |
Enzyme Dilution Buffer |
V773A | 2 × 1.5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
ATP Detection Substrate |
V363C | 1 × 1 vial | |
Nucleotide Detection Buffer |
V696A | 1 × 5ml | |
UDP-Glo™ Enzyme |
V697A | 1 × 20μl | |
UDP, 10mM |
V698A | 1 × 100μl | |
UDP-GA, 100mM |
V732A | 1 × 50μl | View Product |
Enzyme Dilution Buffer |
V773A | 1 × 1.5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
ATP Detection Substrate |
V363A | 1 × 1 vial | |
Nucleotide Detection Buffer |
V696B | 1 × 10ml | |
UDP, 10mM |
V698A | 1 × 100μl | |
UDP-GA, 100mM |
V732A | 1 × 50μl | View Product |
UDP-Glo™ Enzyme |
V697B | 1 × 40μl | |
Enzyme Dilution Buffer |
V773A | 2 × 1.5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
Resources
No related resources available
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Frequently Used With
Ultra Pure UDP-Sugar Substrates
Substrates for use with the UDP-Glo™ Glycosyltransferase Assay.
V7071, V7072, V7081, V7082, V7091, V7092, V7171, V7172, V7321, V7322
GloMax® Explorer System
Easy-to-use, flexible multimode detection system.
GM3500, GM3510
GloMax® Discover System
High-performance microplate reader for detecting luminescence, fluorescence and absorbance.
GM3000
GloMax® Navigator System
Dedicated microplate luminometer.
GM2000, GM2010
